Effect of Incorporating Titanium Dioxide Nanoparticles into White Portland Cement, White Mineral Trioxide Aggregate, and Calcium Enriched Mixture Cement on the Push-out Bond Strength to Furcal Area Dentin.

Statement of the Problem: Bond strength of furcation repair materials is an essential factor in clinical success. Studies on the effect of adding titanium dioxide (TiO2) nanoparticles on the push-out bond strength of commonly used endodontic cements for furcation perforation repair is limited. Purpose: This study aimed to evaluate the effect of adding TiO2 nanoparticles to white Portland cement (PC), white mineral trioxide aggregate (MTA), and calcium enriched mixture cement (CEM) on their push-out bond strengths. Materials and Method: In this in vitro study, 120 endodontically treated molars were assigned to six groups (n=20) based on the material used to repair the perforation. In three groups, the cements (white PC, white MTA, and CEM) were placed in pure form, and in the three remaining groups, 1 weight % of TiO2 was added. The push-out bond strength was measured using a universal testing machine at a strain rate of 0.5 mm/min. Data were analyzed using one-way ANOVA and post hoc Games-Howell test (p< 0.05). Results: One-way ANOVA showed significant differences in the mean bond strength values between the six groups (p= 0.002). The post hoc Games-Howell test showed that the bond strengths in MTA+TiO2 and PC+TiO2 groups were significantly higher than those in MTA and PC groups, respectively. However, there was no significant difference in the bond strength between CEM and CEM+ TiO2 groups. Conclusion: The incorporation of TiO2 into MTA and PC increased their push-out bond strength. However, it did not affect the push-out bond strength of CEM cement.

Effect of different application strategies of universal adhesive used for immediate and delayed dentin sealing on the microtensile bond strength of self-adhesive resin cement to dentin with and without aging.

Background: Bond strength of indirect restorations is a very important issue that should be given special attention. Immediate dentin sealing (IDS) technique has been suggested in recent years. The aim of the present study was to investigate the effect of different strategies of universal adhesive application for immediate and delayed dentin sealing (DDS) on the microtensile bond strength (µTBS) of self-adhesive resin cement with and without aging. Material and Methods: In this experimental study, 24 healthy human third molars were selected. After exposing the occlusal dentin, the teeth were randomly divided into two groups of 12 based on the All-Bond Universal adhesive application strategy (etch-and-rinse or self-etch). Each group was further subdivided into two (n=6) based on IDS or DDS technique. Then composite blocks were cemented on the occlusal surface with self-adhesive resin cement. After cutting the samples into 1 mm2 cross-sections, half of the samples of each subgroup were subjected to µTBS test after one week, and the other half were tested under µTBS after 10,000 thermal cycles. Data were analyzed using three-way ANOVA (P<0.05). Results: µTBS was significantly affected by all three factors of bond strategy, sealing technique and aging. There was also a significant interaction between the three factors. Conclusions: Immediate dentin sealing improved µTBS. The etch-and-rinse strategy resulted in higher µTBS, while aging led to a decrease in µTBS. Key words:Adhesives, dental bonding, dentin, universal, sealing.

Effect of at-home and in-office bleaching on microleakage of class V composite resin restorations using different types of universal adhesives: An in vitro study.

Background: When bleaching agents contact dental structures, they act on restorative materials and adhesive interfaces. This study investigated the effect of "at-home" and "in-office" bleaching on the microleakage of composite resin restorations performed with different universal adhesives in self-etch and etch-and-rinse modes. Methods: Class V cavities were prepared in 132 premolars. The samples were divided into four groups (n=33). All Bond Universal adhesive was used in the first and second groups, and G-Premio Bond adhesive was used in the third and fourth groups. The total-etch mode was used in the first and third groups, and the self-etch mode was used in the second and fourth groups. The samples were divided into three subgroups (n=11). In the first subgroup, home bleaching was used, and in the second subgroup, office bleaching was used. In the third subgroup, bleaching was not performed. The specimens were examined under a stereomicroscope for microleakage. Ordinal regression analysis was applied (P<0.05). Results: The adhesive type, application method, and margin type significantly affected microleakage (P<0.05). The amount of microleakage in All Bond Universal adhesive was significantly higher than in G-Premio Bond adhesive. The chance of microleakage in the self-etch mode was almost twice as high as in the etch-and-rinse mode. The bleaching method did not significantly affect microleakage (P>0.05). Conclusion: Based on the results of the microleakage test, bleaching after composite resin restorations did not significantly affect the microleakage of Class V restorations.

Effect of intraradicular reinforcement strategies on the fracture strength of endodontically treated anterior teeth with overflared canals.

BACKGROUND: This study is done to evaluate the effect of different reinforcement techniques of overflared root canals with fiber posts on the fracture resistance of endodontically treated anterior teeth. It also introduces a new technique for simultaneous reinforcement and cementation of fiber posts using dual-cured core build-up composite resin. MATERIAL AND METHODS: The crowns of 48 single-rooted maxillary anterior teeth were cut and randomly divided into 4 groups (n=12) based on root reinforcement techniques after root canal treatment and post space preparation: Group 1: self-adhesive resin cement (SARC), Group2: dual-cure composite resin (DCC), Group3: Composite resin reinforcement (CRR), Group 4: Direct anatomical post (DAP). Then a core was made for the roots. The periodontal ligament was simulated using a thin layer of PVC impression material. A universal testing machine applied a compressive force to the lingual surface at an angle of 135 ° and 1 mm/min speed. Data were analyzed using One-Way ANOVA and a post-hoc Tukey test. Fracture patterns were analyzed using the Chi-square test (p<0.05). RESULTS: The difference between the control and DAP group was statistically significant (p=0.01), but there were no significant differences between other groups (p>0.05). Regarding fracture patterns, there were no significant differences between groups. CONCLUSIONS: All reinforcement techniques increased the fracture strength compared to the control group. However, DAP was the only group that had a statistically significant difference. CRR and DCC presented intermediate values without significant differences. Key words:Fiber post, endodontically treated teeth, root canals, reinforcement, fracture strength.

Comparison of the effect of preheating on the flexural strength of giomer and nanohybrid composite resin.

Background. Considering the increased use of preheating and novel resin-based materials to restore teeth, the present study investigated the impact of preheating on the flexural strength of a giomer and compared it with a nanohybrid composite resin. Methods. Two restorative materials (Beautifil II giomer and Alpha III nanohybrid composite resin) were used. Thirty rod-shaped samples (adding up to 60 samples) were prepared from the materials above and divided into two subgroups: with and without preheating (n=15). Before sample preparation, the giomer and nanohybrid composite resin tubes were preheated at 68ºC for 15 minutes in the preheating subgroups. In the subgroups without preheating, the tubes were kept in a room at 25ºC. Then the flexural strength was compared between the two groups with two-way ANOVA at a significance level of P<0.05. Results. The results showed significantly higher flexural strength in the preheated subgroups than in the non-preheated subgroups (P<0.001). In addition, the mean flexural strength values were significantly higher in the giomer groups than in the nanohybrid composite resin groups (P<0.001). Conclusion. Preheating increased the studied materials' flexural strengths significantly. The flexural strength of the giomer restorative material was higher than that of the nanohybrid composite resin, irrespective of preheating.

The effects of different surface contaminators on the shear bond strength of a universal adhesive system to dentin: an experimental study.

Background. Contamination of dentin surface is one of the common problems in restorative dentistry. The aim was to investigate the effects of different surface contaminators on the dentin shear bond strength (SBS) of universal adhesive system (UAS) applied in etch-and-rinse (ER) and self-etch (SE) strategies. Methods. One hundred forty-four maxillary anterior sound human teeth were divided into six groups based on the types of surface contaminators: no surface contaminator (control) and experimental groups contaminated with blood, saliva, aluminium chloride (ALC), ferric sulphate (FS), and caries disclosing agent (CDA). Then, each group was further subdivided into two, based on the application strategy of UAS (ER and SE). After applying the adhesive according to the manufacturer's instructions, and bonding cylindrical composite samples, the SBS was measured. The data were analysed using two-way ANOVA, Tukey's HSD test and t test (P < 0.05). Results. The SBS in all contaminated groups, except for CDA, was significantly lower in both ER and SE strategies compared to control group (P < 0.05). A comparison between the application strategies revealed that ER and SE were only significantly different in the FS contaminated group (P < 0.05). Conclusion. All tested contaminators, except CDA, significantly decreased SBS of UAS in both ER and SE strategies.

Effect of accelerated aging and double application on the dentin bond strength of universal adhesive system.

BACKGROUND: Despite the many advantages of simplified adhesive systems, there are concerns about the durability of the adhesive layer over time. The aim was to investigate the effects of various aging methods and double application of an adhesive layer on the bond strength of the universal adhesive system using etch-and-rinse (ER) and self-etch (SE) strategies. MATERIALS AND METHODS: In this in vitro study, the occlusal enamel of 120 extracted, intact human third molars was removed to expose the dentin. Then, the samples were randomly divided into four groups of thirty according to All-Bond Universal (ABU) adhesive application strategy (ER and SE) and the number of adhesive layers (1 or 2). Then, each group was subdivided into three subgroups of ten according to aging method (control, thermal cycling, and 10% sodium hypochlorite [NaOCl]). The shear bond strength was measured at the strain rate of 0.5 mm/min. Data were analyzed using three-way ANOVA and Tukey's post hoc tests (P < 0.05). RESULTS: The effect of adhesive application strategy (P < 0.001) and aging method (P < 0.001) on the bond strength was statistically significant, but the effect of the double application was not statistically significant (P > 0.05). In addition, the interactive effect of adhesive application strategy-aging method was significant (P = 0.005). CONCLUSION: Using ABU with ER strategy leads to higher dentin bond strength compared to the SE method in the control and thermal cycling groups. However, no significant differences were observed between ER and SE strategies after aging with the NaOCl. Furthermore, the double application might not have any effect on the bond strength and durability.

Effect of RaCe, ProTaper, and V-Taper rotary systems on dentinal crack formation during endodontic treatment: An in vitro study.

Background. Prevention of dentinal crack formation is of utmost importance in endodontic treatment. This study aimed to evaluate the effect of RaCe, ProTaper, and V-Taper rotary systems on dentinal crack formation in three root regions during endodontic treatment. Methods. Seventy human mandibular first molars were selected randomly, and their distal roots were used. Ten samples were assigned to the control group, and sixty samples were assigned to three groups (n=20). Each group was prepared with RaCe, ProTaper, or V-Taper rotary files according to the manufacturers' instructions. A stereomicroscope was used to view cracks at ×40 magnification. Friedman's, chi-squared, and Kruskal-Wallis tests were used for statistical analysis of data (P < 0.05). Results. A comparison of the three rotary systems did not reveal any significant differences in the number of cracks between the three root thirds evaluated (P > 0.05). A comparison of the number of cracks in the coronal, middle, and apical thirds in each rotary system showed that the number of cracks in the middle third was fewer than that in the two other thirds only in the V-Taper group (P < 0.05), with no significant differences in the ProTaper and RaCe groups between the different root regions (P > 0.05). Conclusion. The application of RaCe, ProTaper, and V-Taper rotary files resulted in a similar rate of crack formation in endodontic treatment. V-Taper files created the minimum number of cracks in the middle third.

Influence of Varying Dentin and Enamel Layer Thicknesses of Nano-Composite Resins on Color Match between Lithium Disilicate Dental Ceramic and Composite Resins.

Objectives: The aim of this study was to evaluate the influence of varying dentin and enamel layer thicknesses of two nano-composite resins on color match of composite resins and lithium disilicate dental ceramic. Materials and Methods: Twenty-six specimens of two types of nano-composite resins, Opallis and Vittra, were fabricated using the two-layered technique with different thickness ratios of enamel and dentin composites (A2 shade) with a total thickness of 1.2mm. Thirteen discs of the same shade and thickness of IPS e.max Press LT (low translucency) lithium disilicate dental ceramic were also fabricated. Specimen color was measured with a spectrophotometer. The difference in color (ΔE00) of composite and ceramic specimens, and the translucency parameter (TP) of all specimens were calculated. Data were analyzed using multi-factor ANOVA (P<0.05). Results: The color difference (ΔE00) values of composites and ceramic were not clinically acceptable in any areas of either of the two composites (ΔE00>2.25). But ΔE00 between the two composite resins was in the clinically acceptable range (ΔE00<2.25). The mean TP value of IPS e.max Press was greater than that of Vittra and lower than that of Opallis. Conclusion: In similar thicknesses, composite resins with any enamel/dentin thickness ratio could not successfully simulate the color and translucency of IPS e.max Press LT ceramic.

Effect of curing method and thermocycling on flexural strength and microhardness of a new composite resin with alkaline filler.

BACKGROUND: Cention N has been introduced as an alternative material for amalgam. The purpose was to investigate the flexural strength and microhardness of this material in self-cure and dual-cure modes before and after thermocycling. MATERIALS AND METHODS: In this experimental study, 40 samples of Cention N were prepared in order to determine and compare the microhardness. Half of the samples were set by self-cure method and the other half with dual-cure method. The Vickers microhardness test was performed once after 24 h and again after 10000 thermocyclings. Three-point flexural test was used to determine and compare the flexural strength of 52 rod-shaped samples. Half of the samples were set by self-cure method and the other half with dual-cure method. Among 26 samples in each group, 13 samples were randomly selected and three-point flexural test was performed after 24 h and for another 13 samples after 10,000 thermocyclings. Data were analyzed using two-way ANOVA and paired samples t-test (P < 0.05). RESULTS: There was a statistically significant difference between the mean of microhardness values in two curing methods (P < 0.001) and in two storage conditions (P < 0.001). The mean of dual-cure microhardness (100.99 ± 7.22) was higher than that of self-cure (64.61 ± 12.51) and the mean value associated with pre-thermocycling (89.75 ± 15.84) was higher than that of the post-thermocycling (76.44 ± 23.56). There was no statistically significant difference between the mean flexural strength in the two curing methods (self-cure [72.85 ± 16.26], dual cure [79.87 ± 23.07]; [P > 0.05]). However, the mean flexural strength without thermocycling (85.98 ± 21.74) was higher than that of the thermocycled group (64.24 ± 6.40) (P < 0.001). CONCLUSION: The microhardness of Cention N in dual-cure mode was higher than that of self-cure mode, but the flexural strength of dual-cure was not significantly different from that of self-cure. Thermocycling had a significant effect on the microhardness and flexural strength.

Effect of Bleaching Method and Curing Time on the Surface Microhardness of Microhybrid Composite Resin.

Objectives: The present study aimed to evaluate the effect of curing time and bleaching agents on microhybrid composite resin surface microhardness. Material and method: A total of 180 microhybrid composite resin disks were divided into two groups in terms of curing time: 40 s, 60 s. Then, each group was divided into three subgroups: control (distilled water), home bleaching (15% carbamide peroxide) and office bleaching (40% hydrogen peroxide). Surface microhardness of the samples was determined by using Vickers hardness test both at baseline and after the completion of the tests. Two-way ANOVA and Tukey HSD tests were used to analyze and compare microhardness changes between groups. Statistical significance was defined at P<0.05. Results: Based on the two-way ANOVA, curing time, bleaching method, and cumulative effect of these two variables significantly affected composite resin microhardness (P<0.001). Tukey HSD tests showed that microhardness had significantly decreased in the bleaching groups, with the highest decrease when the office bleaching method was used (P<0.001). The samples cured for 40 s exhibited lower microhardness than those cured for 60 s and had an increased reduction in microhardness after bleaching (P<0.001). Conclusions: Application of both bleaching methods decreased the surface hardness of microhybrid composite resin. An increase in the curing time was associated with a decrease in adverse effects of bleaching agents on microhardness.

The effect of pre-heating on monomer elution from bulk-fill resin composites.

BACKGROUND: The present study was aimed to evaluate the effect of pre-heating of bulk -fill resin composites on monomer elution from them. MATERIAL AND METHODS: Three different types of resin composites were used including Tetric N-Ceram Bulk Fill, X-tra Fill and X-tra Base. 10 cylindrical samples were prepared from each resin composites. Before light curing, 5 samples were pre-heated until reaching 68˚C, then 5 other samples were polymerized at room temperature. After 24 hours, release of UDMA, TEGDMA and BIS-GMA monomers were measured by High-Performance Liquid Chromatography analysis. Data analysis was performed by two-way ANOVA test, Games-Howell and Sidak post hoc tests. RESULTS: Pre-heating did not have any statistically significant effect on the mean values of UDMA, TEGDMA and Bis-GMA elution (p>0.05). The greatest amount of released Bis-GMA and UDMA was obtained from Tetric N-Ceram Bulk-fill composite. The greatest amount of released TEGDMA was obtained from X-tra Fill composite. X-tra Base composite showed the lowest amount of monomer release (P<0.001). CONCLUSIONS: Pre-heating did not have any effect on monomer release from bulk-fill resin composites. Moreover, the amount and the type of monomers released from various bulk-fill resin composites were not similar. Key words:Bulk fill composite resin, elution, HPLC, residual monomer, temperature.

Comparison of the effect of bleaching with 15% carbamide peroxide and 35% hydrogen peroxide on flexural strength of Cention N in selfcured and dual-cured polymerization modes.

Background. The use of bleaching agents might result in microstructural changes in tooth structure and in restorative materials. This study compared the effects of bleaching with %15 carbamide peroxide and %35 hydrogen peroxide on the flexural strength of Cention N restorative material using the self-cured and dual-cured polymerization modes. Methods. Sixty bar-shaped samples of Cention N restorative material were included in this in vitro study and assigned to three groups (n=20) randomly: control, bleaching with %15 carbamide peroxide and bleaching with %35 hydrogen peroxide. Each group was divided into two subgroups: samples polymerized in the self-cured mode and samples polymerized in the dual-cured mode. Then the flexurals trengths of the samples were determined. Two-way ANOVA was used to compare flexural strengths between the three groups in two polymerization modes, followed by post hoc Tukey test. Statisticals ignificance was defined at P<0.05. Results. The difference in the mean flexural strength was significant in terms of the bleaching regimen (P<0.001), with significantly lower flexural strength in the two bleaching groups compared to the control group. However, the mean flexural strengths were not significantly different in terms of the polymerization mode applied (P=0.14). Conclusion. The application of %15 carbamide peroxide and %35 hydrogen peroxide bleaching agents decreased the flexural strength of Cention N restorative material. Irrespective of the bleaching regimen, there was no significant difference in the flexural strength of Cention N between the self-curing and dual-curing polymerization modes.

Effect of preheating on the cytotoxicity of bulk-fill composite resins.

Background. Due to the effect of pre-heating on the degree of conversion of composite resins and the possible effect on cytotoxicity, the effect of pre-heating of bulk-fill composite resins was investigated on cytotoxicity in this study. Methods. In this study, three different types of composite resin were used, including Tetric N-Ceram Bulk-Fil, Xtrafil, and Xtrabase. From each composite resin, 10 cylindrical samples (5 mm in diameter and 4 mm in height) were prepared, with five samples preheated to 68°C, and the other five samples polymerized at room temperature (25°C). Twenty-four hours after polymerization, cytotoxicity was assessed by MTT assay on human fibroblasts. Statistical analysis of data was carried out with two-way ANOVA and Sidak Post-Hoc. The significance level of the test was determined at 0.05. Results. There was no statistically significant difference between the mean percentage of cytotoxicity in terms of pre-heating (P>0.05), but the cytotoxicity of the studied composite resins was significantly different (P<0.001). The cytotoxicity of Tetric N-Ceram Bulk-fil composite resin was higher than that of the two other composite resins. Conclusion. Pre-heating of bulk-fill composite resin did not affect their cytotoxicity. In addition, the cytotoxicity of different bulk-fill composite resins was not the same.

Effect of Cavity Disinfection with Chlorhexidine on Marginal Gap of Class V Composite Restorations Bonded with a Universal Adhesive Using Self-Etch and Etch-and-Rinse Bonding Strategy.

OBJECTIVES: This in-vitro study aimed to evaluate the effect of cavity disinfection with chlorhexidine (CHX) on marginal gaps of Class V composite resin restorations bonded with a universal adhesive using self-etch and etch-and-rinse bonding strategy. MATERIALS AND METHODS: Sixty sound human premolars were randomly assigned to two groups (n=30): group 1 (CHX) and group 2 (no CHX). Each group was divided into two subgroups (n=15) according to the bonding strategy of the universal adhesive (self-etch or etch-and-rinse). Class V cavities were prepared on the buccal surfaces of the teeth. The occlusal and gingival margins of the cavities were placed in enamel and dentin, respectively. In the first and second subgroups of both groups, the All-Bond Universal adhesive was applied with self-etch and etch-and-rinse bonding strategy, respectively. After restoration and thermocycling, the samples were sectioned, and marginal gaps at the gingival margins were measured in micrometer (µm) under a stereomicroscope. Two-way analysis of variance (ANOVA) was used to compare marginal gaps between the groups and the subgroups. RESULTS: The mean marginal gap size was significantly affected by cavity disinfection (P=0.001) and bonding strategy (P=0.002). However, the interaction effect of these two factors on the mean marginal gap size was not significant (P=0.79). CONCLUSION: The use of CHX resulted in larger marginal gaps at the gingival margins of Class V composite resin restorations. Irrespective of disinfection, the self-etch bonding strategy resulted in larger marginal gaps compared to the etch-and-rinse bonding strategy.

Correction to: Comparison of the effect of bleaching with 15% carbamide peroxide and 35% hydrogen peroxide on flexural strength of Cention N in self-cured and dual-cured polymerization modes.

[This corrects the article DOI: 10.34172/joddd.2020.023.].

Antibacterial activity of self-adhesive resin cements against Streptococcus mutans at different time intervals.

BACKGROUND AND OBJECTIVES: Self-adhesive resin cements release fluoride and have cytotoxic and preventive monomers against the bacteria in their composition. They have acidic property before their complete setting too. The antibacterial activity of three different self-adhesive resin cements against Streptococcus mutans at different time intervals was investigated in this study. MATERIALS AND METHODS: The modified direct contact test was used to evaluate the antibacterial effect of Max-Cem, G-Cem and Bis-Cem on S. mutans after aging the samples in phosphate-buffered saline solution for one hour, 24 hours and 1 week. Data were analyzed using one-way ANOVA, repeated measurement ANOVA and Tukey HSD tests (P<0.05). RESULTS: The differences in the mean bacterial counts between all the study groups and between the study groups and the corresponding control groups were significant at 1-hour and 24-hour intervals (P<0.001). At 1-week, only the differences between Bis-Cem and G-Cem, between Max-Cem and Bis-Cem, and between Bis-Cem and the corresponding control group were significant (P<0.001). There were significant differences between G-Cem and Max-Cem at all the time intervals (P<0.001). In addition, with the use of Bis-Cem there were significant differences between 1-hour and 1-week (P=0.01) and 24-hour and 1-week (P<0.001). CONCLUSION: All the cements exhibited antibacterial activity after 1 hour and 24 hours. However, after 1 week, only Bis-Cem retained its antibacterial activity.

Surface Roughness of Different Composite Resins after Application of 15% Carbamide Peroxide and Brushing with Toothpaste: An In-Vitro Study.

OBJECTIVES: This study aimed to evaluate the effect of toothbrushing after application of 15% carbamide peroxide (CP) on the surface roughness of three types of composite resins. MATERIALS AND METHODS: Twenty samples, measuring 4 mm in height and diameter, were fabricated of three composite resins namely microfilled (MF) Heliomolar HB, nanohybrid (NH) IPS Empress Direct and microhybrid (MH) Tetric Ceram HB. After polishing, the initial surface roughness was measured with a profilometer. The tray technique was used to apply 15% CP gel for 6 hours. Then, cleaning was carried out with an Oral-B electric toothbrush for 3 minutes in a tank containing a freshly mixed toothpaste. These procedures were repeated for 21 days. Then, the surface roughness was measured again and compared with the initial values. A mixed-design ANOVA model was used for the analysis of data (P<0.05). RESULTS: The baseline roughness was significantly lower in MF compared to the NH and MH composites (P<0.001). Roughness increased in all study groups during the intervention period; however, this increase was not significant in the MH group (P=0.17). Furthermore, the increase in roughness in MF was smaller than that in NH (P<0.001) and MH (P=0.02) groups. CONCLUSION: The effect of intervention was more pronounced on NH and MH groups. Surface roughness changes were minor in MF composite resin.

Effect of bleaching protocols on surface roughness and biofilm formation on silorane-based composite resin.

BACKGROUND: Knowledge about the effect of bleaching on behavior of composite resins is important to find a suitable composite resin for restoration of teeth undergoing bleaching. This study aimed to assess the effect of different bleaching protocols on surface roughness and biofilm formation on a silorane-based composite resin. MATERIALS AND METHODS: In this in vitro experimental study, 60 silorane-based composite resin samples measuring 3 mm in thickness and 6 mm in diameter were fabricated and polished. They were then randomly divided into four groups (n = 15). In Group 1, samples were stored in distilled water as control. Samples in Groups 2, 3, and 4 were subjected to bleaching with 15% carbamide peroxide, 35% hydrogen peroxide, and 35% hydrogen peroxide activated by light, respectively. Surface roughness was measured using a profilometer. Streptococcus mutans cultured in brain-heart infusion broth was used for the assessment of biofilm formation on the samples. The bacterial colonies were counted using the pure-plate technique. Data were analyzed using one-way ANOVA and post hoc Tukey's tests. Regression model was used to assess the association between surface roughness and biofilm formation (P < 0.05). RESULTS: The mean surface roughness of the four groups was not significantly different (P = 0.11); however, a significant difference was noted in the mean biofilm formation among the groups (P = 0.00). CONCLUSION: Bleaching decreased biofilm formation. The lowest biofilm formation was noted in the group subjected to light-activated 35% hydrogen peroxide. Increased surface roughness enhanced biofilm formation to a certain level; excessive roughness did not increase biofilm formation.

Color agreement between try-in paste and resin cement: Effect of thickness and regions of ultra-translucent multilayered zirconia veneers.

Background . The current study aimed at identifying the color agreement between try-in pastes and the respective resin cements and investigated the effect of thickness and regions of Ultra-Translucent Multilayered Zirconia Veneers. Methods . A total of 90 cubic zirconia discs were prepared at two different thicknesses (0.5 mm and 0.7 mm) (n=45) in five groups in terms of the shade of the try-in paste and resin cement as follows: Universal, Clear, Brown, White and Opaque. Try-in paste and the respective resin cement were applied between the specimens and composite substrate, respectively, and colorimetric evaluation was carried out using CIE-Lab system. For each specimen, ΔE between the try-in paste and cement was calculated. Data were analyzed with SPSS 17 using Multifactor ANOVA (P<0.05). Results . Multifactor ANOVA results showed that ΔE values were significantly affected by the resin cement shade and the thickness of ceramic veneer (P<0.05). The results showed better shade agreement between the try-in paste and the respective resin cement with thicker ceramic veneers. The results of Tukey HSD revealed that ΔE values for the Clear, Universal and Brown shades were less than those of the White and Opaque shades. Lighter shades exhibited better agreement between the try-in paste and the respective resin cement. Conclusion . Perceptible color difference was found between the try-in pastes and the respective resin cement in most colors investigated. Although, the agreement of the try-in pastes and the respective resin cement was affected by the thickness of zirconia veneers, the different regions of multilayered ultra-translucent zirconia ceramic showed no significant effect.